Résumés
Abstract
Seasonal variation in relative Blueberry scorch virus (BlScV) concentration was determined for three infected highbush blueberry, Vaccinium corymbosum, cultivars in a commercial field in southwestern British Columbia, Canada. Relative virus concentration per g of infected blueberry flower clusters and leaf terminal tissue varied during the season with significant cultivar-by-time interactions. Flower clusters had the highest BlScV concentration per g of tissue and could be collected in early May for disease surveys. Timing of leaf sample collection for BlScV surveys, transmission studies and virus purification should be based on studies of temporal variation in BlScV concentration for the principal cultivars in a production area.
Keywords:
- Blueberry scorch virus,
- DAS-ELISA,
- highbush blueberry,
- virus concentration
Résumé
Les variations saisonnières dans la concentration relative du virus de la brunissure nécrotique du bleuet (Blueberry scorch virus; BlScV) ont été déterminées pour trois cultivars infectés du bleuetier géant, Vaccinium corymbosum, dans un champ commercial du sud-ouest de la Colombie-Britannique, Canada. La concentration relative du virus par g de grappes de fleurs et de tissu terminal de feuille infectés a varié au cours de la saison, avec des interactions significatives entre les cultivars et le temps. Les grappes de fleurs avaient la plus haute concentration du virus par g de tissu et pouvaient être cueillies au début de mai pour évaluer la maladie. Le moment choisi pour la collecte des échantillons de feuille pour l’étude du BlScV, les études de transmission et le traitement du virus devrait se fonder sur des études de variation temporelle dans la concentration du BlScV pour les principaux cultivars d’une zone de production.
Mots clés:
- concentration virale,
- bleuetier géant,
- test ELISA sandwich,
- virus de la brunissure nécrotique du bleuet
Parties annexes
References
- Bachand, G.D., and J.D. Castello. 1998. Seasonal pattern of Tomato mosaic tobamovirus infection and concentration in red spruce seedlings. Appl. Environ. Microbiol. 64 : 1436-1441.
- Bristow, P.R., R.R. Martin, and G.E. Windom. 2000. Transmission, field spread, cultivar response, and impact on yield in highbush blueberry infected with Blueberry scorch virus. Phytopathology 90 : 474-479.
- British Columbia Ministry of Agriculture and Lands. 2007. Berry Production Guide for Commercial Growers. Lower Mainland Horticulture Improvement Association, Abbotsford, BC, Canada.
- Cavileer, T.D., B.T. Halpern, D.M. Lawrence, E.V. Podleckis, R.R. Martin, and B.I. Hillman. 1994. Nucleotide sequence of the carlavirus associated with blueberry scorch and similar diseases. J. Gen. Virol. 75 : 711-720.
- Ciuffo, M., D. Pettiti, S. Gallo, V. Masenga, and M. Turina. 2005. First report of Blueberry scorch virus in Europe. Plant Pathol. 54 : 565.
- Clark, M.F., and A.N. Adams. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay (ELISA) for the detection of plant viruses. J. Gen. Virol. 34 : 475-483.
- Dal Zotto, A., S.F. Nome, J.A. Di Rienzo, and D.M. Docampo. 1999. Fluctuations of Prunus necrotic ringspot virus (PNRSV) at various phenological stages in peach cultivars. Plant Dis. 83 : 1055-1057.
- Dovas, C.I., A.P. Mamolos, and N.I. Katis. 2002. Fluctuations in concentration of two potyviruses in garlic during the growing period and sampling conditions for reliable detection by ELISA. Ann. Appl. Biol. 140 : 21-28.
- Lowery, D.T., M.G. Bernardy, R.M. Deyoung, and C.J. French. 2008. Identification of new aphid vector species of Blueberry scorch virus. J. Entomol. Soc. B.C. 105 : 27-33.
- MacDonald, S.G., and R.R. Martin. 1990. Survey of highbush blueberries for scorch viruses. Can. Plant Dis. Surv. 70 : 96.
- Martin, R.R., and P.R. Bristow. 1988. A Carlavirus associated with blueberry scorch disease. Phytopathology 78 : 1636-1640.
- Pataky, J.K., C.C. Block, P.M. Michener, L.M. Shepherd, D.C. McGee, and D.G. White. 2004. Ability of an ELISA-based seed health test to detect Erwinia stewartii in maize seed treated with fungicides and insecticides. Plant Dis. 88 : 633-640.
- Polák, J. 1995. Reliability of detection and relative concentration of Plum pox virus determined by ELISA in an infected peach tree during the vegetative period. Z. Pflanzenkr. Pflanzenschutz. 102 : 16-22.
- Polák, J. 1998. Relative concentration of Plum pox virus in leaves and flowers of some Prunus species and cultivars. Acta Virol. 42 : 264-267.
- Raworth, D.A. 2004. Ecology and management of Ericaphis fimbriata (Hemiptera: Aphididae) in relation to the potential for spread of Blueberry scorch virus. Can. Entomol. 136 : 711-718.
- Raworth, D.A., and D. Schade. 2006. Life history parameters and population dynamics of Ericaphis fimbriata (Hemiptera: Aphididae) on blueberry, Vaccinium corymbosum. Can. Entomol. 138 : 205-217.
- Raworth, D.A., C.-K. Chan, R.G. Foottit, and E. Maw. 2006. Spatial and temporal distribution of winged aphids (Hemiptera: Aphididae) frequenting blueberry fields in southwestern British Columbia and implications for the spread of Blueberry scorch virus. Can. Entomol. 138 : 104-113.
- Raworth, D.A., C.J. French, D.T. Lowery, M.G. Bernardy, M. Bouthillier, S. Mathur, C.-K. Chan, R.G. Foottit, E. Maw, L.A. Wegener, and M. Sweeney. 2008. Temporal trends in the transmission of Blueberry scorch virus in British Columbia, Canada. Can. J. Plant Pathol. 30 : 345-350.
- Remaudière, G., and M. Remaudière. 1997. Catalogue of the world’s Aphididae. INRA, Paris, France. 473 p.
- Sutula, C.L., J.M. Gillett, S.M. Morrissey, and D.C. Ramsdell. 1986. Interpreting ELISA data and establishing the positive-negative threshold. Plant Dis. 70 : 722-726.
- Torrance, L., and C.A. Dolby. 1984. Sampling conditions for reliable routine detection by enzyme-linked immunosorbent assay of three ilarviruses in fruit trees. Ann. Appl. Biol. 104 : 267-276.
- Varveri, C., R. Holeva, and F.P. Bem. 1997. Effect of sampling time and plant part on the detection of two viruses in apricot and one in almond by ELISA. Ann. Inst. Phytopathol. Benaki 18 : 25-33.
- Wegener, L.A., R.R. Martin, M.G. Bernardy, L. MacDonald, and Z.K. Punja. 2006. Epidemiology and identification of strains of Blueberry scorch virus on highbush blueberry in British Columbia, Canada. Can. J. Plant Pathol. 28 : 250-262.