Abstracts
Résumé
Le présent travail reporte l’effet du cadmium (Cd), du benzo[a]pyrène (B[a]P) ainsi que leur mélange (Cd/B[a]P), à 1 µM, sur les activités d’enzymes impliqués dans la phase I et la phase II de biotransformation chez le polychète Nereis diversicolor en fonction du temps (après 12, 24, 36 et 48 h).
L’effet d’une contamination aiguë par du cadmium à une dose de 1 µM après 12, 24 et 36 h montre une inhibition de l’activité NADPH cytochrome C réductase chez les individus contaminés comparés à leurs témoins relatifs, alors que le seul effet du cadmium sur l’activité glutathion-S-transférase n’est enregistré qu’après 36 h d’exposition. Quant au benzo[a]pyrène, les résultats montrent une augmentation significative de l’activité NADPH cytochrome C réductase après 12, 24 et 36 h d’exposition, alors que pour l’activité glutathion-S‑transférase, la variation significative entre les animaux témoins et traités n’est enregistrée qu’à 36 h d’exposition. Le mélange (Cd/B[a]P) inhibe l’activité NADPH cytochrome C réductase chez les individus traités par comparaison aux témoins relatifs et montre un effet inducteur sur l’activité GST sauf après 36 h d’exposition. Ces résultats montrent ainsi les interactions entre les polluants ainsi que leurs effets sur les organismes.
Mots clés:
- Nereis diversicolor,
- cadmium,
- benzo[a]pyrène,
- NADPH cytochrome C reductase et glutathion-S-transferase
Abstract
The acute effects of cadmium and benzo[a]pyrene, individually and as a mixture, were evaluated on the phase I and phase II biotransformation enzymes of Nereis diversicolor (Polychaeta) collected from unpolluted sites. In the acute assay (1 µM) after 12, 24, 36 and 48 h, the comparison between worms exposed to cadmium and their controls showed an inhibited activity of NADPH cytochrome C reductase after 12, 24 and 36 h. For the activity of glutathione-S-transferase (GST), however, inhibition in the presence of Cd was only noted after 36 h of exposure. Although exposure to benzo[a]pyrene resulted in a significant increase in NADPH cytochrome C reductase activity after 12, 24 and 36 h, a significant difference in glutathione-S-transferase (GST) activity between treated and control animals was only observed after 36 h. The mixture (Cd/B[a]P) seemed to inhibit the activity of NADPH cytochrome C reductase and to induce the activity of GST in individuals but only up until 36 h of exposure. These findings illustrate the interactions between pollutants and their effects on organisms.
Keywords:
- Nereis diversicolor,
- cadmium,
- benzo[a]pyrene,
- NADPH cytochrome C reductase and glutathione-S-transferase
Appendices
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