Abstracts
Abstract
Root-knot nematode is a major constraint to tomato cultivation in open and protected structures. Resistance sources need to be continuously identified for combating pathogens affecting the yield. In the present studies, forty-seven genotypes of tomato were evaluated phenotypically along with their genotypic characterization. On the basis of their phenotypic reaction, the genotypes were grouped into four categories viz.: resistant, moderately resistant, susceptible and highly susceptible. Of these genotypes, only five were found to be resistant while forty-two were rated from moderately resistant to highly susceptible. Multiplication of Meloidogyne incognita was greatly reduced (Rf < 1) in resistant genotypes as compared to susceptible genotypes. Root galling index was also very low in resistant genotypes. Using molecular markers, the presence of the Mi-1.2 resistance gene was also confirmed in five resistant genotypes (L-0272, NR-14, L-097, L-0275 and PNR-7). These resistant sources could become a source of germplasm in breeding programs for the development of resistant cultivars.
Keywords:
- tomato,
- screening,
- Mi gene,
- resistance,
- Meloidogyne incognita
Résumé
Le nématode à galles est une contrainte majeure à la culture de la tomate dans des structures ouvertes et protégées. Les sources de résistance doivent être identifiées en permanence pour lutter contre les agents pathogènes affectant le rendement. Jusqu’à présent, quarante-sept génotypes de tomate ont été évalués phénotypiquement, de même que leur caractérisation génotypique. Selon leur réaction phénotypique, les génotypes ont été regroupés en quatre catégories : résistant, modérément résistant, sensible et très sensible. Parmi ces génotypes, seuls cinq se sont révélés résistants tandis que quarante-deux ont été classés de modérément résistants à très sensibles. La multiplication de Meloidogyne incognita était fortement réduite (Rf < 1) dans les génotypes résistants par rapport aux génotypes sensibles. L'indice de galles racinaires était également très faible dans les génotypes résistants. À l'aide de marqueurs moléculaires, la présence du gène de résistance Mi-1.2 a également été confirmée dans cinq génotypes résistants (L-0272, NR-14, L-097, L-0275 et PNR-7). Ces sources résistantes pourraient devenir une source de matériel génétique dans les programmes de sélection pour le dévelop-pement de cultivars résistants.
Mots-clés :
- tomate,
- criblage,
- gène Mi,
- résistance,
- Meloidogyne incognita
Appendices
REFERENCES
- Ammiraju, J.S.S., J.C. Veremis, X. Huang, P.A. Roberts, and I. Kaloshian. 2003. The heat-stable root-knot nematode resistance gene Mi-9 from Lycopersicon peruvianum is localized on the short arm of chromosome 6. Theor. Appl. Genet. 106: 478-484.
- Anonymous. 2019. Tomato. Pages 33-41 in S.K. Thind, and J.S. Mahal (eds.), Package of practices for cultivation of vegetables. Punjab Agricultural University, Ludhiana, India.
- Begum, K., N. Hasan, S. Khandker, F.M. Aminuzzaman, M. Asaduzzaman, and N. Akhtar. 2014. Evaluation of brinjal cultivars (Solanum Melongena) against root-knot nematode Meloidogyne spp. Appl. Sci. Rep. 7: 129-134.
- Bozbuqa, R., H.Y. Dasgan, Y. Akhoundnejad, M. Imren, O.C. Gunay, and H. Toktay. 2020. Effect of Mi gene and nematode resistance on tomato genotypes using molecular and screening assay. Cytol. Genet. 54: 154-164.
- Cobb, N.A. 1918. Estimating the nematode population of a soil. Agricultural Technology Circular, Bureau of Plant Industry, Department of Agriculture, USA. 48 pp.
- Danso, Y., R. Akromah, and K. Osei. 2011. Molecular marker screening of tomato, (Solanum lycopersicum L.) germplasm for root-knot nematodes (Meloidogyne species) resistance. Afr. J. Biotechnol. 10: 1511-1515.
- den Ouden, H. 1958. A new method for culturing plants enabling observation of nematodes on growing roots. Eur. J. Plant Pathol. 64: 269-272.
- El-Sappah, A.H., M.M. Islam, H.H. El-awady, S. Yan, S. Qi, J. Liu, G. Cheng, and Y. Liang. 2019. Tomato natural resistance genes in controlling the root-knot nematode. Genes. 10. .
- Foolad, M.R., and D.R. Panthee. 2012. Marker-assisted selection in tomato breeding. Crit. Rev. Plant Sci. 31: 93-123. .
- Goggin, F.L., V.M. Williamson, and D.E. Ullman. 2001. Variability in the response of Macrosiphum euphorbiae and Myzus persicae (Hemiptera: Aphididae) to the tomato resistance gene Mi. Environ. Entomol. 30: 101-106.
- Indiastat. 2019. Socio-economic statistical information about India. Available online [http://www.indiastat.com] (Accessed in December 2019).
- Karssen, G., and M. Moens. 2006. Root-knot nematodes. Pages 59-90 in R.N. Perry, and M. Moens (eds.), Plant nematology. CABI publishing.
- Khan, M.R. 1994. Nematology in developing countries; India-IMP, Region VIII. Pages 379-398 in C.C. Carter, and J.N. Sasser (eds.), An advanced treatise on Meloidogyne vol. 1: Biology and control. Co-publication of Department of Plant Pathology North Carolina State University and the USAID, Raleigh, NC, USA.
- Lodhi, M.A., G. Ye, N.F. Weeden, and B.I. Reisch. 1994. A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Mol. Biol. Rep. 12: 6-13.
- Milligan, S.B., J. Bodeau, J. Yaghoobi, I. Kaloshian, P. Zabel, and V.M. Williamson. 1998. The root knot nematode resistance gene Mi from tomato is a member of the leucine zipper, nucleotide binding, leucine-rich repeat family of plant genes. Plant Cell. 10: 1307-1319.
- Peng, D., and W. Tang. 2001. Advance of resistance gene Mi to root knot nematodes in tomato. J. Shenyang Agric. Univ. 32: 220-223.
- Seah, S., A.C. Telleen, and V.M. Williamson. 2007. Introgressed and endogenous Mi-1 gene clusters in tomato differ by complex rearrangements in flanking sequences and show sequence exchange and diversifying selection among homologues. Theor. Appl. Genet. 114: 1289-1302.
- Seid, A., C. Fininsa, T. Mekete, W. Decraemer, and W.M.L. Wesemael. 2015. Tomato (Solanumlycopersicum) and root-knot nematodes (Meloidogyne spp.) – a century-old battle. Nematology. 17: 995-1009.
- Shurtleff, M.C., and C.W. Averre. 2000. Diagnosing plant disease caused by plant parasitic nematodes. The American Phytopathological Society, St-Paul, MN, USA. 187 pp.
- Taylor, A.L., and J.N. Sasser. 1978. Biology, identification and control of root-knot nematodes (Meloidogyne spp.). Department of Plant Pathology, North Carolina State University, United States Agency for International Development, Raleigh, NC, USA.
- Zakaria H.M., A.S. Kassab, M.M. Shamseldean, M.M. Oraby, and M.M.F. El-Mourshedy. 2013. Controlling the root-knot nematode, Meloidogyne incognita in cucumber plants using some soil bioagents and some amendments under simulated field conditions. Ann. Agric. Sci. 58: 77-82. .