Abstracts
Abstract
A survey on maize virus diseases was conducted in the Trakya region of Turkey by examining 32 496 and 46 871 plants in 2004 and 2005, respectively. Rates of symptomatic plants were estimated at 3.7 to 63.6%, depending on locations. Biological and serological test results revealed the presence of barley yellow dwarf virus-PAV (BYDV-PAV), maize dwarf mosaic virus (MDMV), sugarcane mosaic virus (SCMV), and Johnson grass mosaic virus (JGMV). One hundred forty-two samples were collected randomly from 6492 symptomatic plants in 2004. Seventy-two out of the 142 samples were infected with MDMV, two were infected with BYDV-PAV, 19 with MDMV and BYDV-PAV, two with MDMV, BYDV-PAV and SCMV, and only one sample contained the four viruses. In 2005, 100 other leaf samples were collected randomly from 11 739 symptomatic maize plants. Serological tests revealed that 50% of the samples were infected with MDMV and SCMV; however, five showed mixed infections of two or three combinations of tested viruses. Individual MDMV, SCMV, BYDV-PAV and JGMV infections were detected in five, three, two and four samples, respectively. Presence of MDMV was confirmed by Western blot analysis and IC-RT-PCR. SCMV was also detected by IC-RT-PCR. This is the first study reporting the detection of SCMV and JGMV on maize plants in Turkey.
Keywords:
- ELISA,
- IC-RT-PCR,
- JGMV,
- maize,
- MDMV,
- SCMV
Résumé
Une enquête sur les maladies virales du maïs a été menée dans la région de Trakya en Turquie avec l’examen, en 2004 et 2005, de respectivement 32 496 et 46 871 plantes. Selon l’endroit, le taux de plantes symptomatiques a varié entre 3,7 et 63,6 %. Les résultats de tests biologiques et sérologiques ont révélé la présence de la souche PAV du virus de la jaunisse nanisante de l’orge (BYDV-PAV), du virus de la mosaïque nanisante du maïs (MDMV), du virus de la mosaïque de la canne à sucre (SCMV) et du virus de la mosaïque du sorgho d’Alep (JGMV). En 2004, 142 échantillons foliaires ont été recueillis aléatoirement sur 6492 plantes présentant des symptômes. Soixante-douze des 142 échantillons étaient infectés par le MDMV, deux étaient infectés par le BYDV-PAV, 19 par le MDMV et le BYDV-PAV, deux par le MDMV, le BYDV-PAV et le SCMV, alors qu'un seul contenait les quatre virus. En 2005, 100 nouveaux échantillons foliaires ont été recueillis aléatoirement de 11 739 plants de maïs présentant des symptômes. Des tests sérologiques ont montré que 50 % des échantillons étaient infectés par le MDMV et le SCMV; cependant, cinq échantillons étaient infectés par des combinaisons de deux ou trois des virus testés. Des infections par un seul virus parmi le MDMV, le SCMV, le BYDV-PAV et le JGMV ont été respectivement détectées dans cinq, trois, deux et quatre échantillons. La présence du MDMV a été confirmée par buvardage Western et par immunocapture suivie de transcription inverse et réaction en chaîne de la polymérase (IC-RT-PCR). Le SCMV a aussi été détecté par IC-RT-PCR. La présente étude est la première à signaler, en Turquie, la présence du SCMV et du JGMV dans le maïs.
Mots clés:
- ELISA,
- IC-RT-PCR,
- JGMV,
- maïs,
- MDMV,
- SCMV
Appendices
References
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